progen61055现货——上海起发
anti-AAV2 (intact particle) mouse monoclonal, A20, lyophilized, purified
抗AAV2（完整颗粒）小鼠单克隆抗体
关键功能
纯化、冻干
小鼠单克隆抗体
适用于斑点印迹、ELISA、ICC和中和测定
与AAV2、AAV2 7m8和AAV3完整颗粒反应
同种型：IgG3
| Quantity | 50 µg |
|---|---|
| Antibody Type | Monoclonal |
| Host | Mouse |
| Isotype | IgG3 |
| Conjugate | Unconjugated |
| Application | Affinity chromatography, Dot blot, ELISA, ICC/IF, IP, Neutralization assay |
| Purification | Affinity chromatography |
| Reactivity | AAV2, AAV2 7m8, AAV3 |
| No reactivity | AAV1, AAV4, AAV5, AAV6, AAV7, AAV8, AAV9, AAV11, AAV12, AAVDJ, AAVrh10, AAVrh74 |
| Storage before reconstitution | 2-8°C until indicated expiry date |
| Storage after reconstitution | 2-8°C |
| Intended use | Research use only |
| Clone | A20 |
| Immunogen | AAV2 capsids |
| Concentration | 50 µg/ml after reconstitution with 1 ml dist. water |
| Formulation | Lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09% sodium azide, 0.5% BSA in PBS buffer, pH 7.4) |
| Binding affinity |
KD value (AAV2) = 2.6E-11 M KD value (AAV3) = <1.0E-12 M |
| Synonym | Adeno-associated virus 2; AAV-2 |
| Tested applications | Tested dilutions |
|---|---|
| Immunocytochemistry (ICC)/ Immunofluorescence (IF) | 1:20 |
| Affinity Chromatography | Assay dependent |
| Dot Blot | 1:500 (0.1 µg/ml; non-denaturing conditions) |
| ELISA | Assay dependent |
| Immunoprecipitation (IP) | 1:5 |
| Neutralization Assay | EC50 ~5 ng/ml (AAV2) and ~3 ng/ml (AAV3) - assay dependent |
用于表征感染的不同阶段，对分析AAV2组装过程非常有用。A20与AAV2和AAV3、空衣壳和全衣壳特异性反应。识别组装衣壳的构象表位，不存在于变性衣壳蛋白和天然但未组装的衣壳蛋白中。该抗体不能用于免疫印迹。
表位定位实验（Wobus等人，2000）确定了四个免疫反应（不连续）区域。主要反应归因于AAV2衣壳的AA369至aa378序列。该抗体也可用于中和实验。
Wobus，C.E.等。针对腺相关病毒2型（AAV-2）衣壳的单克隆抗体：参与AAV-2细胞相互作用和中和AAV-2感染的衣壳结构域的表位定位和鉴定。J.维罗尔。74, 9281-93 (2000).
Moskalenko，M.等。人类抗腺相关病毒2型中和抗体的表位定位：对Ge的意义
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